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KMID : 0614019940100010095
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Journal of Pharmaceutical Sciences (C.N.U.)
1994 Volume.10 No. 1 p.95 ~ p.99
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Simultaneous determination of ibuproxam and ibuprofen in human plasma by HPLC with column switching
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E. S Jung
H. S. Lee/J. K. Rho and K. I. Kwon
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Abstract
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A new high-performance liquid chromatographic method with column switching has been developed for the simultaneous determination of Ibuproxam and its metabolite Ibuprofen in plasma. The plasma samples were injected onto a LiChrosorb RP-8 precolumn after dilution with an internal standard solution in phosphoric acid. Polar plasma components were eluted with 0.05 M phosphoric acid. After valve switching, the concentrated drugs were eluted in the back-flush mode and separated on an Inertsil ODS-2 column with acetonitrile-methanol-phosphate buffer as mobile phase. The method showed excellent precision, accuracy and speed with detection limit of 0.1㎍ ㎖^-1. Total analysis time per sample was less than 30 min and the coefficients of variation for intra and inter-assay were less than 3.5%. This method has been successfully applied to plasma samples from human volunteers after oral administration of Ibuproxam.
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KEYWORD
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